RNA/DNA/Protein Mini spin kit

Maximum output – in one procedure NucleoSpin® TriPrep

Simultaneous extraction of

Total RNA

Genomic DNA

Total protein

from one unsplit sample

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NucleoSpin ® TriPrep

Parallel isolation of RNA, DNA, and protein for most significant gene expression profiling

One Prep – Three results

Total RNA of superior quality – for reliable RT-PCR and all common downstream applications Genomic DNA of high purity – ready-to-use for e.g. PCR, sequencing, restriction enzyme digestion T otal protein in high recovery – directly suitable for quantification, SDS-PAGE, and Western blot analysis Rely on your experiments RNA/DNA/protein extracted from one unsplit sample – direct correlation – no variations Save your time

Parallel isolation of RNA, DNA, and protein within one hour

Save precious sample material

Maximum output – isolate three analytes from one sample High sensitivity – ideal for small and limited samples

Procedure

homogenization

of sample

cell lysis

filtration of lysate NucleoSpin bind DNA/RNA

Protein in flow-through

Protein purification

precipitate protein (Protein Precipitator)

wash protein pellet redissolve pellet in Protein Solving Buffer

total Protein

RNA purification digest residual DNA (rDNase incubation at RT)wash RNA elute RNA

DNA purification

wash DNA elute DNA

total DNA DNA bound to silica membrane RNA bound to silica membrane total RNA

Sample Material Sample Amount Lane Human cells (HeLa) 106 cells 1Mouse liver 3 mg 2Fish (Zebrafish) 1 larvae 3Plant root 100 mg 4Plant leave 100 mg 5Yeast 108 cells 6Bacteria 109 cells 7

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Product at-a-glance

Technology

Silica-membrane technology Sample material ≤ 5 x 106 cells

≤ 30 mg human/animal tissue

≤ 100 mg plant tissue Typical yield DNA ≤ 6 μg RNA ≤ 70 μg

Protein ≤ 1,200 μg Typical Ratio A 260/A 280 DNA 1.7 – 1.9

RNA 1.9 – 2.1 Preparation time RNA/DNA/protein ~ 60 – 75 min RNA/DNA ~ 45 min

Protein ~ 35 min

Application data

RNA/DNA/protein extraction from a large variety of starting materials

RNA, DNA, and pr otein were isolated in parallel from the same source. The range of starting materials

covers tissues, cells, plant materials, yeast, and bacteria.

Total DNA of high molecular weight and purity DNA was eluted in 100 μl DNA Elute buffer. A 260/A 280 ratios are in the range of 1.81 – 1.94.

DNA analyzed on 1% TAE agarose gel electrophoresis*

III; Fermentas), Lanes 1-7: see table below

Total RNA of high structural integrity

RNA was eluted in 50 μl RNase-free H 2O.

The RNA integrity number (RIN) was measured for all mammalian samples.RIN was 9.5 for HeLa cell RNA and 8.9 for mouse liver RNA.

RNA analyzed on Agilent 2100 Bioanalyzer/RNA 6000 Nano Kit*

Lane L: RNA Ladder (RNA 6000 Nano Marker; Agilent), Lanes 1-7: see table below

L 1 2 3 4 5 6 7

Protein Solving Buffer .

The proteins are ready to use in SDS-PAGE analysis, as well as for Lane L: Protein ladder (low molecular weight marker; GE), Lanes 1-7: see table below

* Figures are compiled from different gels and runs, aligned to the corresponding length markers

High quality RNA, DNA, and protein

from the same source

Perfectly suitable for gene expression profiling

L 1 2 3 4 5 6 7

NucleoSpin ® TriPrep

Ordering information

Preps

TriPrep*

10/50/250 Mini spin kit for the simultaneous isolation of total RNA, genomic DNA, and total protein from a wide variety of unsplit samples.

Protein Quantification Assay

50/250 RNA/Protein

10/50/250 Mini spin kit for the simultaneous isolation of total RNA and Protein from unsplit samples. Including rDNAse and shredders.

Mini spin columns – XS design

RNA XS

10/50/250 Mini spin kit for the isolation of highly concentrated total RNA from extremely small amount of starting material. Elution down to 5 μl.

RNA/DNA Buffer set*

100 Buffer set for the simultaneous isolation of RNA and DNA from unsplit samples. To be used in combination with NucleoSpin ® RNA II, RNA Plant, NucleoSpin ® RNA/Protein kits.

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Accurate and reliable

Protein concentration down to 0.001 μg/μl Correlation coefficient of 0.97 – 1.00

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